Description
Presence of trace amounts of contaminating genomic DNA (gDNA) in RNA preparations may lead to significant problems, such as false-positive signals and misjudgment of gene expression levels, hence the effective removal of gDNA prior to cDNA synthesis is essential to ensure reliable results.
Xpert cDNA Synthesis Supermix is an optimized mastermix containing a balanced concentration of oligo(dT) and random hexamer primers, dNTPs, and Xpert Reverse Transcriptase (RNase H-). In this way, the need for multiple component additions is eliminated and thus the chance of handling errors is reduced, rendering excellent reproducibility and absolute convenience. The Xpert cDNA Synthesis Supermix already includes gDNA eraser, for efficient elimination of any residual DNA (ssDNA, dsDNA and plasmid DNA) from the RNA sample that otherwise may lead to misjudgment of gene expression levels. Moreover, the Supermix also already includes GRiSP´s RNase inhibitor, which has an improved resistance to oxidation in comparison with human RNase inhibitors and is stable under even very low concentrations of DTT, making it an excellent choice for RNA protection.
In summary, this system provides an efficient and fast method for the synthesis of high-quality, full-length (up to 8-10kb) first strand cDNA with excellent yields from purified poly(A)+ mRNA or total RNA templates.