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Xpert Fast Hotstart DNA Polymerase (with dNTPs)

For fast amplification of complex targets Xpert Fast Hotstart DNA polymerase is a robust enzyme, suitable for the amplification of difficult targets with extreme speed (4-8kb/min), yield and specificity.

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Description

Main application: fast amplification of complex targets

Xpert Fast Hotstart DNA polymerase is a robust enzyme, suitable for the amplification of difficult targets with extreme speed (4-8kb/min), yield and specificity.
The buffer composition has been optimized via high-throughput screening and makes the enzyme particularly resistant to PCR inhibitors. As a result, Xpert Fast Hotstart DNA Polymerase is suitable for direct PCR from unpurified samples, including blood, urine, and bacterial colonies, making this the ideal choice for consistent results in fast complex PCR amplifications.

Additional information

Quantidade

1 ml, 250 U, 5x 1ml, with Dye (400rxn) 5x 1mL, with dye (80rxn) 1mL

Features

Performance highlights:
– Fast amplification of complex targets (including GC/AT-rich)
– Direct PCR from crude samples

Properties:
Amplicon size:  up to 5kb
Extension Rate: 4-8 kb/min
Hotstart:           Yes
A-overhang:      Yes

Applications:
– Direct PCR
– Fast amplification of complex targets (including GC-rich and AT-rich templates)
– Colony PCR
– Multiplex PCR

Components:
– Kit version with dNTPs included (in the buffer)
– Mastermix available with (red) dye, and without dye

Storage:-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles.

Documents

Product Info – Xpert Fast Hotstart DNA Polymerase (with dNTPs)

Product Info – Xpert Fast Hotstart Mastermix

Product Info – Xpert Fast Hotstart Mastermix with dye

Order Information

Reference Description Quantity
GE03.0500 Xpert Taq DNA polymerase 500 U
GE03.2500  Xpert Taq DNA polymerase 2500 U
GE13.0500 Xpert Taq DNA polymerase (+ dNTPs) 500U (+dNTPs)
GE13.2500 Xpert Taq DNA polymerase (+ dNTPs) 2500U (+dNTPs)
GE04.0100 Xpert Taq 2x Mastermix (100rxn) 1.25 ml
GE14.5100  Xpert Taq 2x Mastermix (500rxn) 5 x 1.25 ml
GE14.0100 Xpert Taq 2x Mastermix with dye (100rxn) 1.25ml
GE14.5100 Xpert Taq 2x Mastermix with dye (500rxn) 5 x 1.25 ml

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Product: Xpert Fast Hotstart DNA Polymerase (with dNTPs)

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FAQs

How to use Xpert Fast Hotstart DNA Polymerase (with dNTPs):
Functionally tested in PCR. Absence of endonucleases, exonucleases, and ribonucleases was confirmed by appropriate assays.