Xpert Fast HRM

Xpert Fast HRM (2X mastermix) contains all components, except specific primers and template, for High Resolution Melt analysis in qPCR.


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High Resolution Melt (HRM) analysis is a powerful tool for the detection of mutations and polymorphisms in dsDNA samples, including GpG methylation differences. At increasing temperature, in the presence of a fluorescent dye, tiny sequence variation between samples results in different DNA strand dissociation behaviour. HRM analysis exploits these differences using the melt curve shapes to distinguish between these samples.

Xpert Fast HRM (2X mastermix) contains all components, except specific primers and template, for HRM analysis in qPCR. The mix consists of the combination of a highly efficient enzyme with an optimized buffer system, which allows for efficient amplification of GC-rich and AT-rich templates under both normal and fast qPCR conditions. The intercalating dye used in this mastermix causes no inhibition of the PCR reaction thus allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products.

Different SNPs show different Tm melt curve shifts. Class I SNPs (C/T and G/A) demonstrate typically large shifts (>0.5ºC), whereas Class IV SNP (A/T) typically have very small shifts (<0.2ºC). The high sensitivity and specificity of Xpert Fast HRM allows to distinguish all possible SNPs.

High Resolution Melt (HRM) Analysis
SNP Genotyping
CpG methylation analysis (by comparing bisulfite-treated and control DNA)



Excellent signal with low PCR inhibition
Ultra-sensitive melt profiles
Allows for standard and fast cycling

Xpert Fast HRM is supplied in multiples of 1mL, for your convenience!

One ml of mastermix (2X), containing an optimized ratio of a fast DNA polymerase, dNTPs, MgCl2, and non-PCR inhibiting intercalating green dye, is sufficient for 100 rxns of 20μl.

-20ºC for at least 1 year


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GE70.0100 Xpert Fast HRM 1mL
GE70.5100 Xpert Fast HRM 5x 1mL
GE70.2501 Xpert Fast HRM 25x 1mL