GRiSP

Xpert Fast SYBR (Blue)

For high performance, fast cycling, qPCR with SYBR – with visual aid! Xpert Fast SYBR consists of the combination of a highly efficient enzyme with a novel low inhibitory technology, allowing for extremely high sensitivity and specificity. Includes an inert blue dye as visual aid for pipetting.

 

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Description

Main application: extremely high sensitivity and specificity qPCR with SYBR
This mastermix includes an inert blue dye as visual aid for pipetting.

Xpert Fast SYBR (uni) 2X mastermix contains all components, except primers and template, for the amplification and detection of DNA in qPCR. It consists of the combination of a highly efficient enzyme with a novel low inhibitory technology. The intercalating dye used in this mastermix causes little to no inhibition of the PCR reaction thus allowing for extremely high sensitivity and specificity, as well as preventing the formation of unwanted primer-dimers and non-specific products.

Xpert Fast SYBR (uni) can be used to quantify virtually any DNA target, including extremely low-copy number targets, with minimal effort and optimization.

Xpert Fast SYBR (uni) 2X mastermix is supplied with a separate vial of ROX reference dye, so it can be used with most real-time PCR instruments.

Additional information

Quantidade

1mL (100rxn), 25mL (2500rxn), 5mL (500rxn)

Features

Properties:
– Excellent signal with low PCR inhibition
– Early Ct values – Rapid extenstion rate
– Extreme sensitivity – increased limit of detection
– Allows for standard and fast cycling
– Compatible with all qPCR machines (see FAQs for compatibility)
– 
Includes an inert blue dye as visual aid for pipetting.

Applications:
– Absolute quantification
– Gene expression analysis
– High throughput qPCR
– Low-copy number target genes

Performance:
 

Amplification and melt trace of ACTG1 (Actin Gamma-1). Comparison between Xpert Fast SYBR (orange), and Market Leader (black).

Components:
Xpert Fast SYBR Blue is supplied in multiples of 1mL, for your convenience!
Xpert Fast SYBR Blue is only available in Uni version (for NoROX, LowROX, or HighROX qPCR machines)

– Xpert Fast SYBR (uni) BLUE
Xpert Fast SYBR (uni) BLUE is supplied as a convenient 2x mastermix containing all required components except specific primers. 1mL is enough for 100 reactions of 20μl.
Supplied also with a separate vial of 100X (50µM) ROX solution. Depending on the PCR equipment (see FAQs), prior to use, one should add 2µl of ROX (LowROX (final concentation of 50nM), 20µl (HighROX (final concentation of 500nM)), or nothing (No ROX). Once ROX has been added, the mastermix might be used immediately or stored at -20ºC.

Storage:
-20ºC for at least 1 year.

Documents

Xpert qPCR selection table

Product Info – Xpert Fast SYBR Blue

Photos

Order Information

Reference Description Quantity
GE22.0100 Xpert Fast SYBR(Uni)BLUE 1ml(100rxns)
GE22.5100 Xpert Fast SYBR(Uni)BLUE 5ml(500rxns)
GE22.2501 Xpert Fast SYBR(Uni)BLUE 25ml(2500rxns)

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Product: Xpert Fast SYBR (Blue)

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FAQs

How to use Xpert Fast SYBR (Blue):
Q: How much ROX should I add to Xpert Fast SYBR (uni) ?
A: This depends on whether your instrument is “No ROX”, “LowROX”, or “HighROX” (see table below), In case of “No ROX”, one should use the mastermix as is, thus without adding additional ROX. In case of “LowROX”, one should add 2µl of 100X ROX solution (supplied) to 1ml of mastermix, and in case of “HighROX”, one should add 20µl of 100X ROX solution to 1ml of mastermix. Once ROX has been added, the mastermix can be used directly or stored at -20ºC for up to 1 year. If your instrument is not listed, please feel free to contact us.

Xpert Fast SYBR Selection Table:

Manufacturer Model No ROX LowROX HighROX
Agilent/Stratagene MX3000P® X
MX3005P® X
MX4000P® X
BioRad CFX96™ X
CFX384™ X
Cromo4™ X
MiniOpticon™ X
Opticon™ X
Opticon™2 X
Illumina Eco™ X
Life Technologies ABI7000 X
ABI7300 X
ABI7500 X
ABI 7500 Fast X
ABI 7700 X
ABI 7900 X
ABI 7900HT X
ABI 7900HT Fast X
QuantStudio™12k Flex X
StepOne™ X
StepOne™Plus X
ViiA7™ X
Qiagen Rotor-Gene™ 3000 X
Rotor-Gene™ 6000 X
Rotor-Gene™ Q X
Roche LightCycler™ 96 X
LightCycler™ 480 X
LightCycler™ nano X
Q: When should I use Xpert Fast SYBR (fluorescein) ?
A: Xpert Fast SYBR (fluorescein) has been developed for instruments equipped with a well-factor correction feature, These include BioRad´s iCYCLER®, IQ™5, and MyiQ™. If your instrument is not listed, please feel free to contact us.