Description
Main application: high-fidelity amplification with enhanced speed and robustness
Xpert HighFidelity DNA polymerase is a robust enzyme with enhanced DNA binding, resulting in improved processivity, yield, and extremely low error-rate, ideal for applications such as high-fidelity PCR, site-directed mutagenesis, crude sample PCR, blunt-end cloning, among others, where robustness and proof-reading are important.
The Xpert HighFidelity DNA polymerase has an error-rate of approximately 1 error per 4.5×107 nucleotides incorporated, which is 50x lower than Taq DNA polymerase.
The velocity of Xpert HighFidelity DNA polymerase allows the use of an extension rate of up to 2kb/min, making this also an ideal choice for consistent results in fast PCR amplifications.