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Xpert One-Step Fast Probe

Using gene-specific primers (GSP), Xpert One-Step Fast Probe allows for first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. Xpert One-Step Fast Probe consists of a RTase mix, a Fast qPCR Mastermix (Probe), and separated vial(s) of ROX

 

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Description

Using gene-specific primers (GSP), Xpert One-Step Fast Probe allows for first-strand cDNA synthesis and subsequent qPCR in a single-tube reaction procedure, decreasing contamination risk and reducing hands-on time considerably. Xpert One-Step Fast Probe consists of a RTase mix, a Fast qPCR Mastermix (Probe), and separated vial(s) of ROX

The RTase mix comprises a genetically modified thermostable MMLV reverse transcriptase with improved synthesis efficiency, lacking any RNase H activity, and an advanced RNase inhibitor to impede RNA degradation. The Fast qPCR Mastermix (Probe) consists of the combination of a highly efficient enzyme with a novel low inhibitory technology, which prevents the formation of unwanted primer-dimers and non-specific products, thus allowing for extremely high sensitivity and specificity. It can be used in qPCR based on a wide range of probe-based technologies, including Taqman®, Molecular Beacons® and Scorpion probes®.

Xpert One-Step Fast Probe can be used to quantify virtually any RNA target, whether using mRNA, viral RNA or total RNA as template, including extremely low-copy number targets, with minimal effort and optimization.

Additional information

Quantidade

100 rxn, 5x 100 rxn

Features

Applications:
One-Step RT-qPCR,
bsolute quantification,
Gene expression analysis,
Low-copy number target gene detection.

Properties:
Excellent signal with low PCR inhibition
Early Ct values – Rapid extension rate
Allows for standard and fast cycling

Samples:                    
total RNA: 1pg – 1µg, mRNA: >0.01pg
Storage:
-20ºC for at least 1 year. Repeated thaw/freeze cycles (up to 25) have no negative impact on performance.

Documents

Product Info Xpert One-Step Fast Probe

Order Information

Reference Description Quantity
GE50.0100 Xpert One-Step Fast Probe 100 rxn
GE50.5100 Xpert One-Step Fast Probe 5x 100 rxn

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