GRiSP

Xpert Purification Magnetic Beads

Magnetic Beads for the purification of DNA of 100bp and larger. Primers, primer dimers, dNTPs, enzymes, excess salts, and other impurities can be removed quickly and efficiently by a simple washing procedure. Readily adaptable to 96-well or 384-well microplate automation platforms.

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Description

Xpert Purification Magnetic Beads consists of paramagnetic particles coated with carboxyl groups that reversibly bind DNA (solid phase reversible immobilization) . The magnetic beads are supplied in a buffer that has been optimized in order to selectively bind DNA fragments of 100bp and larger. Primers, primer dimers, dNTPs, enzymes, excess salts, and other impurities can be removed quickly and efficiently by a simple washing procedure. Because this purification method does not require centrifugation or vacuum filtration, it can be readily adapted to 96-well or 384-well microplate automation platforms. Moreover, these beads can be seamlessly integrated into NGS Library preparation workflows.

Additional information

Quantidade

25 ml, 5 ml, 60 ml

Documents

Product Info – Xpert Purfication Magnetic Beads

 

Protocols

Basic Protocol During steps 4 to 8 maintain the microplate on the magnetic separation rack at all times.

  1. Add Xpert Purification Magnetic Beads to the DNA samples using a beads to DNA ratio of 1.8
  2. Mix thoroughly to a homogenous appearance by pipetting up and down the entire mixture 10 times. Allow for optimal DNA binding by incubation at room temperature for 5 minutes.
  3. In order to separate the magnetic beads from the solution, place the microplate onto a magnetic separation rack for 5 minutes (Ensure the solution has becomes clear before proceeding to step 4).
  4. Carefully aspirate off the cleared solution and discard. Avoid disturbing the magnetic beads.
  5. Dispense 70% ethanol to each well: 200µl/well in case of a 96-well plate or 30µl/well in case of a 384-well plate.
  6. Incubate at room temperature for 30 seconds.
  7. Carefully aspirate off the ethanol and discard. Avoid disturbing the magnetic beads.
  8. Repeat steps 5-7 once, then allow the plate to air-dry for 3-5 minutes to remove any residual ethanol.
  9. Remove the microplate from the magnetic rack and elute DNA with 10-50µl/well (as desired) of elution buffer (e.g. nuclease-free water, TE, 10mM Tris-HCl pH 8.0 or 10mM Tris-acetate pH 8.0).
  10. Mix thoroughly by pipetting up and down the entire mixture 10 times.
  11. Incubate at room temperature for 2-5 minutes.
  12. Place the microplate back onto the magnetic separation rack for 5 minutes.
  13. Carefully transfer the eluent to a new plate. In case of carryover of magnetic beads, repeat step 12 and transfer into another new plate (or tube).
  14. Store purified DNA at -20ºC or proceed with downstream application.

Order Information

Reference Description Quantity
GK19.0005 Xpert Purfication Magnetic Beads 5ml
GK19.0025 Xpert Purfication Magnetic Beads 25ml
GK19.0060 Xpert Purfication Magnetic Beads 60ml

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Product: Xpert Purification Magnetic Beads

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FAQs

How to use Xpert Purification Magnetic Beads:
PROTOCOL

1) Transfer 5µl of PCR product to a new tube
2) Add 1µl of rSAP and 1µl of Exo I
3) Incubate at 37ºC for 5 minutes
4) Heat-inactivate at 80ºC for 10 minutes

The purified PCR product can be used for DNA sequencing reaction immediately