Legionella is the sole genus of the family Legionellaceae, which consists of over 50 species comprising over 70 serogroups. Nearly half of Legionella species are associated with human disease, such as Pontiac Fever and Legionnaires´ disease (LD). LD is the most severe form of infection, with clinical symptoms including pneumonia and a fatality rate of around 10%. More than half of the isolates associated with LD in previously healthy individuals are L. pneumophila (serogroup1).
Legionella spp. are very common to the aqueous with amoebae being among their natural hosts. Legionella infection occurs via inhalation of water droplets from a contaminated source that has allowed the organism to grow and spread. Legionella spp. can often been found, in high concentration, in a wide variety of environmental water sources such as ponds, hot-water tanks, hot tubs, showerheads, whirlpools, spas, air-conditioning, cooling towers, and public fountains, if those water systems are not managed correctly (e.g. in case of stagnation and warm temperatures).
Traditional microbiological detection and confirmation methods are long established and well developed but are laborious and typically require many days in order to obtain results, as Legionella are slow growing bacteria. This qPCR Detection Kit provides a fast, sensitive and reliable method for the detection of Legionella based on qPCR using specific FAM-labeled probes, requiring only a few hours. This immense time reduction allows taking appropriate action, if needed, much sooner.
This kit is compatible with instruments equipped with FAM and ROX channels. The detection limit is approximately 5pg of Legionella DNA allowing the detection of as little 103 cells/L of water, with a specificity of 100%.