Since 1994, over 100 genetically modified plants have received approval for use as food or animal feedstuff. In most countries, usage of GMOs is highly regulated. For the enforcement of national legislation, efficient detection of genetically modified organisms in food and feed products is essential. Taking into account the large diversity, an initial generic screening for the presence of the most common GM material is usually the first step in GMO analysis, in order to reduce the amount of subsequent identification analysis. As the promotor 35S from cauliflower mosaic virus (CaMV) and the terminator NOS from Agrobacterium tumefaciens are the most frequent elements present in transgenic material found in food and feed, detection of these regulatory sequences by PCR/qPCR amplification is the most logical choice.
The Xpert qDetect P-35S and T-NOS is a kit that allows for the detection by qPCR of DNA sequences from the promotor 35S and/or the terminator NOS present in total DNA previously purified from food or feed samples. This kit is compatible with instruments equipped with FAM and ROX channels. The detection limit is 10-100 pg of GMO DNA, allowing the detection of as little as 0.01-0.1% of target DNA in food samples if 100ng of total DNA (mixed species) is used