Salmonella spp. are a major cause of foodborne illness worldwide. Sources of these facultative intracellular pathogens include poultry, eggs, raw meat, raw milk, fish and shellfish. Cross-contamination of ready-to-eat foods that are not subsequently processed or are undercooked can lead to incidents of food poisoning. Moreover, the presence of Salmonella is potentially prejudicial to health, regardless of the level of contamination, as the infective dose can be quite low (10 to 100 cells), especially if food has high fat content.
Traditional microbiological detection and confirmation methods are long established and well developed but typically require 4-5 days in order to obtain results. This qPCR Detection Kit provides a fast, sensitive and reliable method for the detection of Salmonella spp. based on qPCR using specific FAM-labeled probes, requiring less than 2 days. This immense time reduction allows taking appropriate action, if needed, much sooner.
This kit is compatible with instruments equipped with FAM and ROX channels. The detection limit is approximately 500fg of Salmonella DNA allowing the detection of, after enrichment, as little as 1-10 cells per 25g of food sample, with a specificity of 100%.