Yeast Nitrogen Base w/o added amino acids and with ammonium sulfate

Dehydrated powder for the preparation of YNB liquid medium for the classification of yeast based on their carbon and/or nitrogen requirements.

 

 

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Description

Dehydrated powder for the preparation of YNB liquid medium for the classification of yeast based on their carbon and/or nitrogen requirements

Features

Quantity: 500g
Appearance: Beige powder. Stock solution should be green/yellow.
Storage: 2ºC – 25ºC. When not in use, keep container closed to avoid hydration.

Formulation (g/L)
YNB: 1,70
Ammonium sulfate: 5,00
Final pH (25ºC): 5,4 ± 0,2

Preparation:
Prepare a 10x stock solution by adding 6,7g of the dehydrated medium to 100ml of distilled water, and 5 grams of dextrose (glucose) or equivalent amount of another carbohydrate and 5-10mg of the desired amino acid(s). Mix well and dissolve by heating with regular agitation. Do NOT Boil. Do NOT autoclave. Sterilize the solution by filtration and store at 2ºC to 8ºC.

Usage
Prepare Yeast Nitrogen Base medium by pipetting 0,5ml of the 10x stock solution into 4,5ml of distilled, sterile water. Mix by swirling before inoculation. YNB medium w/o added amino acids and w/o ammonium sulfate contains all the essential ingredients (see table below) that are required for the cultivation of yeast, except for the amino acids, a nitrogen source and a carbohydrate source.

Trace Elements (mg/L)
Inositol……………2,0
Boric Acid. ………………….0,5
Manganese sulfate……0,4
Potassium Iodide………..0,1
Niacin……………….…0,4
Pyridoxine HCL…0,4
ZnSO4……………………….0,4
Sodium molybdate ……0,2
Thiamide HCL………….0,4
Calcium Pantothenate 0,4
Ferric Chloride..…0,2
Biotin ……………………..0,002
Copper Sulfate………0,04
p-Aminobenzoic acid …0,2
Riboflavin……………..0,2
Folic Acid…..…0,002
monopotassium phosphate..1.0g
Calcium Chloride……..0,1g
Sodium Chloride……….0,1g
Magnesium sulfate……0,5g

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GCM16.0500 YNB without amino acids and with ammonium sulfate 500g