Ready-to-use pre-stained protein marker suitable for monitoring protein separation during SDS-PAGE, verification of Western Transfer Efficiency onto membranes and for estimation of the molecular weight of proteins and/or polypeptides in the range of
Ready-to-use pre-stained protein marker suitable for monitoring protein separation during SDS-PAGE, verification of Western Transfer Efficiency onto membranes and for estimation of the molecular weight of proteins and/or polypeptides in the range of 10-180kDa. The GRS Protein Marker Blue is composed of 11 blue bands including 2 reference bands with more intense colour for easy identification (~25kDa and ~72kDa).
Quantity: 500μl of approx. 0.1~0.5 mg/ml of each protein in 20mM Tris-phosphate , pH 7.5 (25°C), 2% SDS, 1mM Dithiothreitol, 3.6M Urea, and 15% (v/v) Glycerol.
Storage: Up to 2 weeks at room temperature, 3 months at +4ºC, 2 years at -20ºC.
Contents (kDa)
10 | 15 | 20 | 25 | 35 | 45 | 60 | 72 | 100 | 140 | 180 (Tris-Glycine 10%)
Recommended Loading
3μl or 5μl for clear visualization during electrophoresis on mini-gel (100-150 lanes).
2μl or 3μl per well for general Western transferring (150-250 lanes).
Apply more for thicker (> 1.5 mm) or larger gels.
| GLP02.0500 | GRS Protein Marker Blue (100 lanes) | 500uL |
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