Ready-to-use pre-stained protein marker suitable for monitoring protein separation during SDS-PAGE, verification of Western Transfer Efficiency onto membranes and for estimation of the molecular weight of proteins and/or polypeptides in the range of
Ready-to-use pre-stained protein marker suitable for monitoring protein separation during SDS-PAGE, verification of Western Transfer Efficiency onto membranes and for estimation of the molecular weight of proteins and/or polypeptides in the range of 10-250kDa. The GRS Protein Marker Multicolour is composed of 12 bands: 10 blue bands and 2 reference bands with different colours for easy identification (one red band (~25kDa) and one green band (~75kDa)).
Quantity: 500μl of approx. 0.1~0.4 mg/ml of each protein in 20mM Tris-phosphate , pH
7.5 (25°C), 2% SDS, 1mM Dithiothreitol, 3.6M Urea, and 15% (v/v) Glycerol.
Storage: Up to 2 weeks at room temperature, 3 months at +4ºC, 2 years at -20ºC.
Contents (kDa)
11 | 17 | 20 | 25 | 35 | 48 | 63 | 75 | 100 | 135 | 180 | 245 (Tris-Glycine 4-20%)
Recommended Loading
3μl or 5μl for clear visualization during electrophoresis on mini-gel (100-150 lanes).
1.5~2.5 μl per well for general Western transferring (200-300 lanes).
Apply more for thicker (> 1.5 mm) or larger gels.
| GLP01.0500 | GRS Protein Marker MultiColour (100 lanes) | 500uL |
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