Xpert AmpliFi DNA Polymerase is an all-round and robust high-fidelity enzyme with enhanced DNA binding, resulting in improved processivity, shorter extension times, higher yield and capacity to amplify more difficult and longer targets. Combined with its extremely low error-rate, this makes Xpert AmpliFi ideal for applications such as high-fidelity PCR, site-directed mutagenesis, cloning, long-range PCR and DNA sequencing, when robustness and proof-reading are extremely important.
Xpert AmpliFi DNA polymerase has been derived from Pfu DNA polymerase for its proof-reading activity. Xpert AmpliFi has a very high accuracy resulting in fidelity that is about 100x higher than regular Taq DNA polymerase. DNA binding has been improved by protein engineering resulting in shorter extension times (2-6kb/min), making this also an ideal choice for consistent results in fast PCR amplifications.