Xpert Green DNA Stain is a new and safe alternative to ethidium bromide (EtBr) for the visualization of DNA (double-stranded and single-stranded DNA) and RNA in agarose and polyacrylamide gels. The dye has been developed for in-gel staining and is compatible with both UV and Blue LED transilluminators.
Xpert Green DNA Stain has 2 fluorescence excitation wavelengths in the UV range (~270nm; ~290nm) and one in the blue light range (~485nm). Maximum fluorescence emission is at ~525nm (green). Therefore, Xpert Green DNA Stain is compatible with a large variety of gel documentation systems. Yellow/orange or green filters should be used for photography.
The detection limit of Xpert Green DNA Stain is in the range of 0.5-5.0 ng/band (depending on agarose type and percentage, thickness of the gel, electrophoresis buffer, transilluminator, photo camera quality and settings, etc). Band intensity can be improved by adding Xpert Green DNA Stain to the electrophoresis buffer, especially in case of small DNA fragments that migrate farther. Xpert Green DNA Stain can be used for post-staining, however, with less sensitivity.
Xpert Green DNA Stain is non-mutagenic as determined by the Ames-test. Moreover, genotoxicity analysis shows negative results for both the mouse marrow chromophilous erythrocyte micronucleus test and mouse spermatocyte chromosomal aberration test. The complete safety report can be found on the document page of this product. Xpert Green DNA Stain is non-hazardous, however, one should always exercise common safe laboratory practices. Use goggles and gloves as Xpert Green DNA Stain may cause skin or eye irritations.
Xpert Green DNA Stain is not classified as hazardous waste and some institutions have approved the disposal of gels and solutions containing Xpert Green DNA Stain directly into their wastewater systems. GRiSP recommends to dispose of Xpert Green DNA Stain as you would of any other non-carcinogenic fluorescent dye (like propidium iodide). Naturally, always dispose in accordance to all Federal, state, and local environmental regulations.
This product is stable for at least a few days at room temperature. Transport is carried out at room temperature. Upon arrival, product can be stored at +2ºC to +8ºC, protected from light, for at least 2 years. Do not freeze. Gently spin down before use.