GRiSP

Xpert Green DNA Stain direct

Xpert Green DNA Stain direct is a new and safe alternative to ethidium bromide (EtBr) for the visualization of DNA (double-stranded and single-stranded DNA) and RNA in agarose and polyacrylamide gels. The dye has been developed for direct loading and is compatible with both UV and Blue LED transilluminators. Xpert Green DNA Stain direct is provided as a sample loading dye. There is no need to add any dye to the gel or running buffer: just add Xpert Green DNA Stain direct to your sample and perform electrophoresis.
Clear

Description

Xpert Green DNA Stain direct is a new and safe alternative to ethidium bromide (EtBr) for the visualization of DNA (double-stranded and single-stranded DNA) and RNA in agarose and polyacrylamide gels. The dye has been developed for direct loading and is compatible with both UV and Blue LED transilluminators. Xpert Green DNA Stain direct is provided as a sample loading dye. There is no need to add any dye to the gel or running buffer: just add Xpert Green DNA Stain direct to your sample and perform electrophoresis.

Properties:
Xpert Green DNA Stain direct has 2 fluorescence excitation wavelengths in the UV range (~270nm; ~290nm) and one in the blue light range (~490nm). Maximum fluorescence emission is at ~515nm (green). Therefore, Xpert Green DNA Stain direct is compatible with a large variety of gel documentation systems. Yellow/orange or green filters should be used for photography.

Sensitivity:
The sensitivity of Xpert Green DNA Stain direct is considerably higher than that of EtBr. The detection limit of Xpert Green DNA Stain direct is in the range of 0.1-1.0 ng/band (depending on agarose type and percentage, thickness of the gel, electrophoresis buffer, transilluminator, photo camera quality and settings, etc).

Safety:
Xpert Green DNA Stain direct is non-mutagenic as determined by the Ames-test. Moreover, genotoxicity analysis shows negative results for both the mouse marrow chromophilous erythrocyte micronucleus test and mouse spermatocyte chromosomal aberration test. The complete safety report can be found on the product page at our website. Xpert Green DNA Stain direct is non-hazardous, however, one should always exercise common safe laboratory practices. Use goggles and gloves as Xpert Green DNA Stain direct may cause skin or eye irritations.

Waste:
Xpert Green DNA Stain direct is not classified as hazardous waste and some institutions have approved the disposal of gels and solutions containing Xpert Green DNA Stain direct directly into their wastewater systems. GRiSP recommends to dispose of Xpert Green DNA Stain direct as you would of any other non-carcinogenic fluorescent dye (like propidium iodide). Naturally, always dispose in accordance to all Federal, state, and local environmental regulations.

Storage:
This product is stable for at least a few days at room temperature. Transport is carried out at room temperature. Upon arrival, product can be stored at +2ºC to +8ºC, protected from light, for at least 2 years. Do not freeze. Gently spin down before use.

 

 

 

Additional information

Quantidade

1 ml

Features

  • Direct Loading (no need for loading dye)
  • Safe (non-carcinogenic, non-mutagenic, non-toxic)
  • For dsDNA, ssDNA, and RNA
  • For Agarose and polyacrylamide gels
  • Compatible with UV Light and Blue Light
  • Ultrasensitive
  • No Hazardous waste
  • Magnificent signal to noise ratio
  • Improved Cloning Efficiency (when using Blue Light)

 

 

Documents

Download Product Info Datasheet

Download Flyer Xpert Green Stains

Download MSDS GS02 Xpert Green DNA Stain Direct v7E502

Safety Report Xpert Green DNA Stains

 

Protocols

Agarose Gels
1. Prepare agarose solution by mixing the desired amount of agarose and desired volume of electrophoresis buffer, and dissolving the agarose by heating.
2. Once the solution has become clear, remove from the microwave or heater, swirl gently, and allow the solution to cool down to ~60?C. Pour to cast the gel.
3. Mix samples (and DNA marker) with Xpert Green DNA Stain direct in a 10:1 ratio (e.g. 10μl of sample with 1μl of Xpert Green DNA Stain direct) and load onto gel. There is no need to add any other loading buffer.
4. Run gel according to normal procedure and visualize using either UV or Blue LED light.

Polyacrylamide Gels
1. Prepare polyacrylamide solution by mixing all components except APS and TEMED.
2. Add APS and TEMED, swirl gently, avoiding formation of air bubbles, and cast the gel.
3. Mix samples (and DNA marker) with Xpert Green DNA Stain direct in a 1’:1 ratio (e.g. 10μl of sample with 1μl of Xpert Green DNA Stain direct) and load onto gel. There is no need to add any other loading buffer.
4. Run gel according to normal procedure and visualize using either UV or Blue LED light.

Order Information

Reference Description Quantity
GS02.0001 Xpert Green DNA Stain Direct 1ml

Ask a Question

Note that by submitting this contact form, you are providing GRISP with some personal information. We encourage you to take the time to review our revised Privacy Policy.

Product: Xpert Green DNA Stain direct

Request Price for Xpert Green DNA Stain direct

FAQs

How to use Xpert Green DNA Stain direct:
Consulte os docs abaixo