Xpert Green DNA Stain direct is a new and safe alternative to ethidium bromide (EtBr) for the visualization of DNA (double-stranded and single-stranded DNA) and RNA in agarose and polyacrylamide gels. The dye has been developed for direct loading and is compatible with both UV and Blue LED transilluminators. Xpert Green DNA Stain direct is provided as a sample loading dye. There is no need to add any dye to the gel or running buffer: just add Xpert Green DNA Stain direct to your sample and perform electrophoresis.
Xpert Green DNA Stain direct has 2 fluorescence excitation wavelengths in the UV range (~270nm; ~290nm) and one in the blue light range (~490nm). Maximum fluorescence emission is at ~515nm (green). Therefore, Xpert Green DNA Stain direct is compatible with a large variety of gel documentation systems. Yellow/orange or green filters should be used for photography.
The sensitivity of Xpert Green DNA Stain direct is considerably higher than that of EtBr. The detection limit of Xpert Green DNA Stain direct is in the range of 0.1-1.0 ng/band (depending on agarose type and percentage, thickness of the gel, electrophoresis buffer, transilluminator, photo camera quality and settings, etc).
Xpert Green DNA Stain direct is non-mutagenic as determined by the Ames-test. Moreover, genotoxicity analysis shows negative results for both the mouse marrow chromophilous erythrocyte micronucleus test and mouse spermatocyte chromosomal aberration test. The complete safety report can be found on the product page at our website. Xpert Green DNA Stain direct is non-hazardous, however, one should always exercise common safe laboratory practices. Use goggles and gloves as Xpert Green DNA Stain direct may cause skin or eye irritations.
Xpert Green DNA Stain direct is not classified as hazardous waste and some institutions have approved the disposal of gels and solutions containing Xpert Green DNA Stain direct directly into their wastewater systems. GRiSP recommends to dispose of Xpert Green DNA Stain direct as you would of any other non-carcinogenic fluorescent dye (like propidium iodide). Naturally, always dispose in accordance to all Federal, state, and local environmental regulations.
This product is stable for at least a few days at room temperature. Transport is carried out at room temperature. Upon arrival, product can be stored at +2ºC to +8ºC, protected from light, for at least 2 years. Do not freeze. Gently spin down before use.