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Xpert TaqPLUS DNA polymerase (with dNTPs)

For long amplifications, and difficult targets Xpert TaqPLUS DNA polymerase is an enhanced DNA polymerase with superior PCR performance when it comes to the amplification of long templates (up to 30kb).

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Description

Main application: amplification of difficult and long targets

Xpert TaqPLUS DNA polymerase is an enhanced DNA polymerase with superior PCR performance when it comes to the amplification of long templates (up to 30kb).
The buffer composition has been optimized via high-throughput screening and the enzyme is particularly efficient for the amplification of difficult templates, such as mammalian genomic DNA, and GC-rich or AT-rich templates. As a result, Xpert TaqPLUS DNA Polymerase is this the ideal choice for consistent results in complex PCR amplifications.

Additional information

Quantidade

1 ml, 250 U, 5x 1ml, with dye (80rxn) 1mL

Features

Performance highlights:
– hotstart enzyme ideal for the amplification of demanding samples, including very long templates

Properties:
Amplicon size:  up to 30kb
Extension Rate: 4 kb/min (up to 5kb, 1 kb/min for larger amplicons)
Hotstart:           Yes
A-overhang:      Yes

Applications:
– Very difficult templates
– Long PCR

Components:
– Kit version with dNTPs included (in the buffer)
– Mastermix available with (red) dye, and without dye

Storage:
20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles

Documents

Datasheet – Xpert TaqPLUS DNA polymerase (with dNTPs)

Datasheet – Xpert TaqPLUS mastermix

Datasheet – Xpert TaqPLUS mastermix with dye

 

Order Information

Reference Description Quantity
GE09.0250 Xpert TaqPlus Hotstart DNA polymerase (with dNTPs) 250U
GE19.0001 Xpert TaqPlus Hotstart 2X mastermix (80rxn) 1mL
GE19.5001 Xpert TaqPlus Hotstart 2X mastermix (400rxn) 5x 1mL
GE29.0001 Xpert TaqPlus Hotstart 2X mastermix with dye (80rxn) 1 mL
GE29.5001 Xpert TaqPlus Hotstart 2X mastermix (400rxn) 5x 1mL

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Product: Xpert TaqPLUS DNA polymerase (with dNTPs)

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FAQs

How to use Xpert TaqPLUS DNA polymerase (with dNTPs):
Functionally tested in PCR. Absence of endonucleases, exonucleases, and ribonucleases was confirmed by appropriate assays.